The Maroulakou Laboratory is interested in the biochemical basis for growth, survival and transformation of mammary epithelial cells. We use biochemical and genetic methods to understand the normal and oncogenic properties of genes implicated in breast cancer, including the Akt gene family Tpl2 and cyclinD1. To this end, we have developed and utilized knockout and transgenic mouse models, with an emphasis on establishing genetic systems that permit the cell-specific and temporal inactivation and reactivation of genes.  Additionally, we utilize these mouse models to develop and study xenograft mouse models, which will be used to explore significant signaling pathways involved in tumor progression as well as genetic changes implicated with these processes.  Through these studies, we intend to contribute towards more effective and strategic cancer treatments by identifying specific therapeutic targets. Our research is focused in the following areas: 1. The study of genes that are associated with breast cancer and the exploration of signaling pathways regulated by them during initiation, progression and metastasis. 2. The role of breast cancer-related genes in the developmental biology of the mammary gland. 3. Functional activities of the breast cancer related genes within the Epithelial and Mesenchymal compartments of the breast . 4. The use of cells derived from genetically engineered mice to study the functions of these genes in cell culture models.